Detection of Mitochondrial DNA T16189C Polymorphism
Journal: International Journal of Science and Research (IJSR) (Vol.5, No. 4)Publication Date: 2016-04-05
Authors : Nihad A.M. Al-Rashedi; Emad Uldeen Hateem;
Page : 1945-1947
Keywords : mtDNA polymorphism; T16189C; PCR-RFLP; DNA Sequencing;
Abstract
This study can be observed as polymorphic nucleotide where T to C transition was observed at the 16189 nucleotide position in mitochondrial DNA. The aim of this study was to confirm to the mtDNA polymorphic nucleotide which may be useful in the forensic genetics and clinical applications. The 100 blood samples of Iraqi population were collected for mitochondrial DNA extraction and evaluated for mtDNA 16189 variant by PCR-RFLP technique. Then, digested fragments were ready to direct DNA sequencing. The results showed that detection of transition T to C at the position 16189 in mitochondrial DNA by PCR-RFLP and direct DNA sequencing. The frequency of transitional polymorphic nucleotides at positions 16189 were 0.16 observed highest. It is transition generates heteroplasmic length tract of polycytosine which has advantage in mitochondrial DNA fingerprinting and index of different metabolic disorders.
Other Latest Articles
- Dynamic Routing for Data Integrity and Delay Differentiated Services in Wireless Sensor Networks
- Ensuring Efficient Data Transmission in Wireless Sensor Networks in Secure Way
- Development and Effects of Jamun Seed Powder Incorporated Cookies
- Economic Analysis of Micro Hydel Plant-A Case Study
- A Cloud based Solution for Online Examination Management in Education-An Architecture
Last modified: 2021-07-01 14:33:56