Theory and Practice of Prion Protein Analysis in Food Products
Journal: "Foods and Raw materials" Journal (Vol.2, No. 2)Publication Date: 2014-12-30
Authors : Prosyekov A.;
Page : 106-120
Keywords : Prion; protein; encephalopathy; safety; quality; PCR; analysis;
Abstract
The article presents the results of the research on methods of identification and quantitative determination of prion proteins in biological samples and multicomponent mixtures based on them. Analysis of nucleotide sequence of DNA encoding the PRNP gene of the prion protein, including phylogenetic and comparative analysis of nucleotide sequences of normal and pathogenic prion protein in cattle, was performed. Oligonucleotide primers for amplification of the PRNP gene of pathogenic prion protein were designed and synthesized. The high specificity of the developed test system was confirmed.
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