APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION
Journal: Biotechnologia Acta (Vol.14, No. 2)Publication Date: 2021-04-30
Authors : N. O Volkova M. S. Yukhta L. V. Sokil L. G. Chernyschenko L. V. Stepanyuk A. M. Goltsev;
Page : 28-36
Keywords : bovine serum albumin; rehabilitation; cryopreservation; fragments of the seminiferous tubules of testes.;
Abstract
An optimal approach to the recovery of testicular tissue fragments after cryopreservation is critical for their further use in order to successful fertility restoration. Aim. The purpose of this study was to investigate the effect of bovine serum albumin (BSA) addition to the rehabilitation medium on the morphofunctional characteristics of fragments of seminiferous tubules of testes (FSTT) of immature rats after cryopreservation. Methods. The object of the study was cryopreserved by slow cooling and vitrified FSTT. Warmed samples were incubated for 30 min in Leibovitz medium supplemented with BSA at concentrations of 0, 2, 5 or 10%. After that, morphological characteristics, the activity of the metabolic and antioxidant systems were evaluated. Results. It was found that in the samples cryopreserved by slow cooling, the use of 5% BSA contributed to the increase in the safety of spermatogenic epithelium cells, in the levels of metabolic and antioxidant activities. In case of vitrified FSTT samples, it was observed that the addition of 5% BSA to the medium promoted the repair of minor tissue damage and increased metabolic activity level, but did not affect the state of the antioxidant defense system. Conclusions. The obtained data can be used to develop an effective rehabilitation medium for cryopreserved fragments of the seminiferous tubules of testes using BSA.
Other Latest Articles
- Pelatihan Pemasaran Syariah Berbasis Digital bagi Pelaku UMKM di Kelurahan MekarsariCileungsi
- TECHNOLOGIES OF GENETIC MATERIAL USE RESTRICTION: TYPES, MOLECULAR-GENETIC BASE AND ETHICAL ANALYSIS OF THEIR APPLICATION
- GIBBERELLINS IN REGULATION OF PLANT GROWTH AND DEVELOPMENT UNDER ABIOTIC STRESSES
- FIRST EXPERIENCE WITH LAPAROSCOPIC RESECTION OF SPLEEN CYST (CASE STUDY AND BRIEF REVIEW OF THE LITERATURE)
- PROGRESSIVE FAMILY INTRAHEPATIC CHOLESTASIS: DIAGNOSTICS, TREATMENT (CLINICAL OBSERVATION)
Last modified: 2021-09-15 23:53:23