Synthesis and Characterisation Of Novel Turmeric Gold Nanoparticles and Evaluation Of Its Antioxidant, Anti-Inflammatory, Antibacterial Activity For Application In Oral Mucositis-An Invitro Study

Objective: This article aimed to green synthesize and characterize turmeric mediated gold nanoparticles (TuAuNP) and to evaluate its antioxidant, anti-inflammatory, antibacterial activity against oral pathogens for its application in oral mucositis. Materials and Method: Aqueous extract of turmeric powder were used to reduce, stabilize and capgold nanoparticle (AuNP). Reduction of Au3+ to Au NPs were initially observed by colour change from yellow solution to red violet colour within 15 minutes. TuAuNP were analytically characterised by UV-visible spectrophotometer (UV-Vis), Transmission Electron Microscopy (TEM), X-Ray Diffraction Assay (XRD), Fourier Transform Infra Red Analysis (FTIR) and evaluated its antioxidant, anti-inflammatory, antibacterial activity against oral pathogens. Results: Surface Plasmon Resonance bandwith an absorption peak at around 540 nm preliminarily confirmed TuAuNP synthesis. TEM Analysis showed spherical shaped, smooth edged, size ranged of 5-15 nm. XRD analysis revealedface centered cubic crystallinity with intense sharp peaks at 38°, 44°, 64° and 77°. FTIR showed peaks of the functional groups of different phytochemicals of the rhizome extract involved as reducing and capping agent. TuAuNp exhibited 83% highest scavenging ability of DPPH radical at concentration of 50 μg/mL. TuAuNP showed 89.5% of maximum anti-inflammatory activity at 50μg/mL than standard drug diclofenac. TuAuNP exhibited good antibacterial activity against Streptococcusmutans, Klebsiella Pneumoniae at 100µg/mL, Pseudomonasaeruginosa, Enterococcifaecalis at 50µg/mL, moderate antibacterial activity against Staphylococci aureus at 50µg/mL. Conclusion: Turmeric mediated green synthesis of gold nanoparticles exhibited good antioxidant, anti-inflammatory, broad spectrumantibacterial activities against oral pathogens that could be applied in treating oral mucositisas oral insitu or transmucosal preparations