Study Effect of Chlorhexidine - Honey and Propolis on Lactobacillus in Children. In Vitro Study

Oral mouthwash are good alternatives to lotions in chemical composition, like chlorhexidine. because of this, there is an increase in interest in natural alternatives such as honey and propolis, which have proven their effectiveness over time. Aim: study effect of oralwashes of honey 50%, chlorhexidine 0.12% and propolis 5% on lactobacillus . Materials and Methods: the sample included 60 syrian children (30 males - 30 females), between (6-12) years. This study continued from September 2020 until February 2021, a saliva smear was taken for each child Before oral washing with research materials and swab after oral rinsing using 10 ml of solution for 30 seconds, then cultivate it in the laboratory where it is grown on agar MRS medium and conducting a count of the results and comparing the averages before and after and the effect of each substance on the lactobacillus bacteria. Results: after one minute of using the mouthwashes, the decrease rate in lactobacillus bacterial colony count was statistically significant in the chlorhexidine mouthwash as it was 80.55%, in the propolis mouthwash it was 77.34%, and in the honey mouthwash it was 61.90%. Conclusion: This study demonstrated the efficacy of oral chlorhexidine, propolis and honey mouthwashes on lactobacillus where the largest decrease effect was in chlorhexidine, then propolis, then honey, This indicates that the three substances are effective in reducing lactobacillus , which is recommended to use them when needed to reduce lactobacillus and to reduce dental caries.