A Comparative Study on Callus Induction and Indirect Morphogenesis in Two Papaveraceae Species

The aim of the study was callus induction of two species of Papaveraceae family (Chelidonium majus L. and Glaucium flavum Crantz) using various types of explants on in vitro media supplemented with different combinations of plant growth regulators (PGRs). Their indirect morphogenetic capacity has been subsequently investigated, too. Entire in vitro seedlings or organs excised from them were used as explants for callus induction. High percentage of seedlings and root explants from G. flavum formed calli in almost all tested combinations of PGRs. In C. majus, the roots were the most appropriate among all tested explant types, followed by seedlings and hypocotyls, regardless of the PGRs used for stimulation of callogenesis. For both studied species, only in the combination with N-(2- Chloro-4-pyridyl)-N'-phenylurea (4-CPPU) no callogenesis was observed. The combination of α-Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) stimulated morphogenetic processes in the seedlings – shoot organogenesis in C. majus and somatic embryogenesis in G. flavum.