EXTRACTION OF CAFFEINE FROM COFFEE & STUDY IT’S EFFECT ON MICROBIAL GROWTH

The research work presented in this paper is Effect on caffeine on the growth of Staphylococcus aureus microorganism. Caffeine is a plant alkaloid which are found in more than 60 plant species just like coffia arabica (Coffee), Thea sinensis (Tea plant).It is plant product that is most commonly found in coffee beans, tea, cocoa and chocolates. Caffeine is also found in some prescription and non-prescription drug including cold, allergy and pain relievers. And it is mostly used by human. In this experiment we extract the caffeine from coffee powder using organic solvent (Ethyl acetate). The effect of caffeine was studied on Staphylococcus aureus. The caffeine proved the result show as Antibacterial agent.