Study of the Antimicrobial Action of Some Commercial Mouthwash (MW) Solutions against Biofilm Formation by Oral Bacterial Microflora

The aim of the study was to evaluate the in vitro antibacterial action of some commercial mouth washing solutions against biofilm formed due to oral microflora from saliva of healthy persons. In this study, four oral mouthwash (MWs) solutions, Listerine 100%, Oral B 100%, Chlorhexidine 0.12%, and Sensodyne 100%, were selected for evaluating the antibacterial activity against biofilm formation. About 10 ml of saliva sample was taken from 5 healthy donors in the morning before eating and brushing their teeth and collected in sterile containers. Half of the collected saliva was filter-sterilized and used for coating and biofilm formation on a 96 ELISA-well microplate, the other part was used to study the antimicrobial activity of MW solutions. Biofilm formation was evaluated with crystal violet methods. Crystal violet-based method was used to determine the amount of biofilm, and optical densities (OD) of the wells in the plate were measured at a wavelength of 600 nm using an ELISA microplate reader to determine the effect of mouthwash solutions. The results showed that the amount of biofilm formed by the samples treated with Listerine, Oral B, Chlorhexidine and Sensodyne was found to be 28.4%, 32.95%, 37.5%, 60.22%, respectively. All mouthwash solutions showed a highly significant reduction in the biofilm amount (P-value = 0.00). Accordingly, in this study, all the commercial MW solutions showed antibiofilm activity, but Oral B was found to be the most effective mouthwash solution against oral biofilm formation.