Isolation of Canine Parvovirus from Rectal Swabs of Suspected Dogs from Various Regions in Northern India

Canine parvovirus (CPV) is a highly contagious pathogen causing acute gastro-intestinal illness among the canine population. The study aimed to isolate canine parvovirus in a Madin- Darby Canine Kidney (MDCK) cell line from rectal swabs of dogs having signs of gastroenteritis. A total of 45 rectal swabs were collected from dogs exhibiting clinical signs of canine parvovirus infection from various regions of northern India, viz., Punjab, Assam, Delhi, Chandigarh and Jammu. The samples were processed and subjected to MDCK cell isolation, and isolates that exhibited cytopathic effects (CPE) like rounding of cells, clumping and detachment of cells were confirmed by PCR and NPCR. Out of 45 samples, 12 (26.66%) isolates showed CPE on MDCK. Further, it was observed that out of these 12 isolates, three isolates were from dogs which had history of vaccination for the disease.