In-vitro Anti - Diabetic Activity and Qualitative Bio Chemical Analysis of 20 MEGATHUKKU KUDINEER - A Siddha Formulation

In recent times, there is a growing interest towards Siddha medicine for the treatment of Diabetes mellitus. 20 Megathukkukudineer (MK) is a Siddha classical polyherbal formulation that has been mentioned in Sarabenthirar Vaithiya Muraigal Neerizhivu Sikitchai for the treatment of diabetes mellitus. The aim of this study is to analyse the in-vitro antidiabetic potential and bio-chemical analysis of 20 Megathukkukudineer. 20 Megathukkukudineer was prepared by boiling the ingredients weighed 20g in 160ml of water and reduced to 20 ml. The test sample (MK) was prepared in the serial dilution of concentrartion ranges from 100, 200, 300, 400 and 500 ?g /ml using DD water. The invitro alpha amylase and alpha glucosidase enzyme inhibition of MK sample was compared with standard drug acarbose and the IC 50 value was calculated. The data was statistically analysed and expressed as Mean +SD ( n =3). The result showed that MK had maximum activity towards the inhibition of enzyme alpha amylase (52.82 ? 17.33) and alpha glucosidase (38.61 ? 2.74) when compared with standard acarbose 98.62 ? 1.655 and 99.15 ? 0.3842 respectively. The qualitative phytochemical test revealed that MK contains calcium, sulphate, chloride, starch, unsaturated compound and amino acid.