The Administration of 35% Chocolate Seaweed (Sargassum polycystum) Extract Cream Does Not Inhibit TGF-?1 Decrease But Inhibit Collagen Decrease in Wistar Rats Exposed to Ultraviolet B

Background: Exposure to UV-B rays is one of the cause of the decrease in TGF-?1 and collagen in the skin. The mechanism of this decrease is caused by the formation of reactive oxygen species. One of the natural ingredients that can be used to inhibit collagen degradation is brown seaweed (Sargassum polycystum). The content of fucoxanthin, flavonoid, phenol and tannin in Sargassum polycystum is believed to be a high source of antioxidants, which can inhibit the decrease in TGF-?1 and collagen due to exposure to UV-B rays. The purpose of this study is to prove that the administration of brown seaweed (Sargassum polycystum) extract cream can inhibit the decrease of TGF-?1 and collagen in Wistar rats exposed to UV-B light. Methods: This research are an experimental study randomized post test only control group design. Samples were 45 male rats (Rattus novergicus) aged 10-12 weeks and weight 200-250 grams. The samples were divided into 3 groups, namely the untreated group, the control group which was only exposed to UV-B light and the treatment group which was given 35% chocolate seaweed (Sargassum polycystum) extract cream 20 minutes before UV-B exposure and 4 hours after. The control group and the treatment group were exposed to UV-B rays with the same total dose of 840 mJ/cm2 for 4 weeks. The research data were then analyzed using the One way Anova test. Results: The results showed that there was no significant difference in the mean level of TGF-?1, where in the untreated group the average was 446.58?57.01 pg/mL, while in the control group the results were 458.11?49.63 pg/mL and in the treatment group it was 478.27?68.41 pg/mL (p=0.319). However, there was a significant difference in the amount of collagen, where in the untreated group was 72.15?4.28%, the control group was 49.16?3.22% and the treatment group was 64.29?4.02% (pless than0.001). Conclusion: From the results of this study, it can be concluded that the administration of chocolate seaweed extract cream did not significantly inhibit the decrease in TGF-?1 levels, but it could significantly inhibit the decrease in the amount of collagen in Wistar rats exposed to UV-B light.