Neuropharmacological Assessment of Dual Herbal Combination Using Zebrafish Model

The goal of neuropharmacological screening is to evaluate new pharmacologically active drugs. The purpose of this study is to look at the neuropharmacological effects of 1:1 combination of flax seed oil and allspice oil in different zebrafish models. Flaxseed is a dietary supplement that can help with constipation, diabetes, high cholesterol, heart disease, cancer, and a variety of other problems. Lignans.antioxidants, fibre, protein, and polyunsaturated fatty acids like alpha-linolenic acid (ALA), or omega-3, are all found in flaxseed. Allspice oil has anticancer. Antifungal, antibacterial, nematicidal, anti-diabetic, anaesthetic, antiseptic, acaridical, and anti-oxidant properties. The zebrafish did not demonstrate behavioural changes or other symptoms of toxicity or mortality after consuming allspice and flax seed, indicating their safety profile. Because of the presence of aromatic oil content the product has significant anaesthetic characteristic in the zebrafish model of anaesthesia. It is crucial to investigate the effects of flaxseed essential oil and allspice oil in the zebrafish Y maze and dark/light paradigm, which showed memory-enhancing and anxiolytic advantages via cholinergic regulation and antioxidant capacities. Individual examination findings did not reveal the same impact in the case of CRC of ACH utilising the combination of dual herbal oil, indicating that they had cholinomimetic effects. Their antioxidant properties and suppression of acetylcholine esterase has shown its potential to be investigated further as a therapeutic drug for Alzheimers disease.