Isolation, Identification and Identification of Medically Important Microorganisms from Poultry Droppings at Selected Poultry Farms in Nnewi, Nigeria

Poultry farming is a very lucrative business across Nigeria. One of the major challenges faced with this farming is transfer of microorganisms from the birds to humans through direct contact, animal danders, environment and poultry droppings. This study aimed at evaluating microorganisms of medical importance found in poultry droppings located at three poultry farms across Nnewi, Nigeria. A 1 g portion of each poultry dropping sample was homogenized in 10 ml buffered peptone water, after which, 0.1 ml of each homogenate was pour plated on nutrient agar, Mac Conkey agar and Sabouraud's dextrose agar. The bacterial isolates were identified using morphological characterization, Gram staining and biochemical tests such as citrate test, methyl red test, indole test and sugar fermentation test. Fungal Isolates were identified with slide culture method followed by fungal atlas identification. Poultry farm C had the most bacterial count in its chicken droppings which is 7.0×106 cfu g while farm B had the highest fungal count of 8.5×104 cfu g. Bacteria isolated were E. coli, Bacillus species and Staphylococcus species, while fungi identified were Aspergillus species and Rhizopus species. This study has been able to show that some medically important microorganisms can be present in poultry farms and thus requires strict hygiene to be observed in poultry farm maintenance. Umeoduagu, N. D. | Dimejesi, S. A. "Isolation, Identification and Identification of Medically Important Microorganisms from Poultry Droppings at Selected Poultry Farms in Nnewi, Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-6 , October 2022, URL: https://www.ijtsrd.com/papers/ijtsrd51929.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/51929/isolation-identification-and-identification-of-medically-important-microorganisms-from-poultry-droppings-at-selected-poultry-farms-in-nnewi-nigeria/umeoduagu-n-d