Impact of Growth Regulators on Rapid Clonal Propagation of Rotala rotundifolia (Buch-Ham. ex Roxb) in Liquid Culture Medium

Rotala rotundifolia (Buch-Ham. ex Roxb) is water plant used for medicinal and ornamental purposes. The aim of the work was to investigate the impacts of different growth regulators on the in vitro clonal propagation of R. rotundifolia. All assays were performed in liquid Murashige and Skoog (MS) media (without agar). Nodal explants were transferred to liquid MS nutrient media. Different combinations of Gibberellic Acid (GA3) + Thidiazuron (TDZ) and Kinetin (KIN) + Gibberellic Acid (GA3) were added to the nutrient solutions as growth regulators. The best results in terms of shoot numbers and shoot lengths were determined in nutrient solution including 0.25 mg/L GA3+ 0.25 mg/L TDZ and 0.25 mg/L KIN+ 0.25 mg/L GA3. When both hormone combinations were compared, higher shoot numbers were found in nutrient solutions including GA3+TDZ. On the other hand, longer shoots were obtained in nutrient solutions including KIN+GA3 After the propagation trials, the plants formed roots in the media with 0.25 mg/L indole-3-butyric acid (IBA). The plants that have been produced have been successfully adapted to the aquarium.