Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis
Journal: Molecular Biology Research Communications (Vol.11, No. 3)Publication Date: 2022.09.01
Authors : Sepehr Valizadeh; Mojtaba Zehtabi; Neda Feiziordaklou; Zahra Akbarpour; Amir Mahdi Khamaneh; Mortaza Raeisi;
Page : 133-141
Keywords : Papillary thyroid cancer; MicroRNA; Bioinformatics; Microarray data analysis;
Abstract
Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.
Other Latest Articles
- The Influence of Martial arts on the Development of Modern Dance
- Cakenjring Banyumas Traditional arts Worth as a Cultural Tourist Object
- Analysis of Organizational learning Obstacles and How to Make Organizations Learn
- Identification of Bordetella bronchiseptica in the throat and nose of dogs and cats by PCR
- I Gusti Ayu Agung Dian Susanthi
Last modified: 2023-02-06 14:57:31