EFFECT OF TEMPERATURE, PRESERVATIVE AND TIME ON THE AFERMENTATION OF URINE AND BLOOD

This study was designed to detect and determine the concentration of ethanol in biological samples (blood and urine). samples were collected from anon drunken, diabetic persons some of them used drugs and other not used and drunken person and divided to four plain containers. Two of them with preservative (Sodium fluoride) and others without preservative and kept at 4°C and at 40 °C for 10 days. Potassium dichromate test (Alco test) was used to detect the presence of ethanol every 24 hours for 10 days. All samples from drunken and diabetic people were negative for ethanol presence. Meanwhile, all samples from drunken persons were positive result and the colour of potassium dichromate changed to green colour indicated the presence of ethanol. In the other hand all samples were positive of ethanol with GC (FID), but they were different in their concentrations. Samples at 40 °C were showed higher concentration than other samples with preservative or at 4°C; therefore, the GC method for analysis ethanol is very important as confirmatory test and to identify the type of alcohol.