Haemoglobin Variant Study by HPLC Method at a Tertiary Care Centre
Journal: Journal of Medicinal and Chemical Sciences (Vol.6, No. 11)Publication Date: 2023-11-01
Authors : Pradnya Phalak; Abhijit Pratap;
Page : 2841-2848
Keywords : haemoglobinopathy; HPLC; Thalassemia; Sickle Cell; Prevalence;
Abstract
Haemoglobinopathies are genetic disorders arising due to defect in the globin chain of haemoglobin. It is relatively common amongst genetic disorders. Beta thalassemia and Sickle cell are two commonest types of haemoglobinopathy encountered and clinically range from mild or asymptomatic condition to life threatening condition requiring blood transfusions for survival. In India, haemoglobinopathies seen are both thalassemias and sickle cell syndromes. The aim of the study is to determine the prevalence of haemoglobinopathies at our tertiary care centre from the haemoglobin sample processed. Accordingly, the study estimated the haemoglobin variant by HPLC method on 100 patients over 3 years and the diagnosis of haemoglobinopathies were confirmed after taking into consideration the results of variant study, haematological parameters and clinical profile of the patients. The results revealed out of 100 patients, 27 patients showed abnormal haemoglobin variant study and the most common haemoglobinopathy observed was 17 cases of beta thalassemia trait, 4 cases of sickle cell trait, 4 cases of sickle beta thalassemia, and 1 case of haemoglobin E trait and beta thalassemia major. The abnormal haemoglobin most commonly prevalent was beta thalassemia trait which was similar to other studies done in India and many subpopulation studies. All haemoglobinopathies were advised family study for evaluation and future genetic implications. Haemoglobin E trait was diagnosed based on the retention time observed as the equipment failed to recognize the abnormal variant. One patient was on regular blood transfusion and was having beta thalassemia major haemoglobinopathy.
Other Latest Articles
- Prevalence of Iron Overload in Patients with Multiple Sclerosis
- Erythema Nodosum: A Manifestation of Trichomoniasis and Vulvovaginal Candidiasis
- Levels of Zinc and Iron Serum in Children with Febrile Seizures: Systematic Review and Meta-Analysis
- Risk of Increased Post-Transfusion IL-8 Levels in Adult Patients with Malignancy Receiving Non-Leukodepleted Packed Red Cell Transfusions
- Comparison of Complication between Autograft and Xenograft Duraplasty: A Systematic Review and Meta-Analysis
Last modified: 2023-07-31 18:50:53