IDENTIFICATION OF MICRORGANISMS BY USING AUTOMATED MICROBIAL DETECTION SYSTEM IN ADULT PATIENTSSUSPECTED WITH SEPTICAEMIA WITH ITS SUPPLEMENTARY PARAMETERS

Septicaemia is a major cause of mortality in both developed and developing countries. Despite important progress in treatment and prevention of infectious diseases, they are considered as leading cause of death and disability. The purpose of this study was monitoring the spectrum of microorganisms that invade blood stream and to study role of different markers in septicaemia, such data are often used to determine empiric antibiotic therapy, to alert clinicians to emerging pathogens that may pose a threat to community and also help in diagnosing severity of septicaemia. Aims and Objectives: To isolate and study the microorganisms from septicaemiccases, to study antimicrobial susceptibility of microorganisms isolated from septicemic casesandto study role of different markers in septicemic cases. Which help in diagnosing severity of septicaemia. Methodology: The study was conducted from September 2015 to February 2017 in a tertiary care teaching hospital. 1993 samples were collected from patients clinically suspected patients of septicaemia. They were inoculated and then incubated, and gram staining was performed. Antibiotic resistance and susceptibility were measured by the disk diffusion method according to the Clinical and Laboratory standards institute (CSLI) guidelines Result:Out of 1993 patients having, 695(34.87%) were culture positive. Among Gram negative isolates, Klebsiella spp.(14.96%)was commonest followed by Acinetobacter species (13.66%) ,E.coli (7.76%),Pseudomonas aeruginosa (6.76%) and salmonella spp.(1.29%). Among Gram positive organisms in our study CONS was the commonest (14.53%) followed by S. aureus (10.79%) and Enterococcus (6.18%), Streptococcus species (1.72%). It shows that colistin was found to be more sensitive for gram negative organisms followed by aminoglycosides and carbapenem group, while resistance was observed towards fluoroquinolone, 1st and 2nd generation cephalosporine groups. &Vancomycin, Teicoplanin and Linezolide were highly active drugs against Gram positive organisms. Conclusion:Septicaemia is an important cause of morbidity and mortality. The study conducted showed both Gram positive and Gram-negative bacteria were responsible for septicaemia. Most of the strains were multi drug resistant and leaving limited options for treatment. Thus, timely detection and knowledge of most likely pathogens causing septicaemia along with their antibiotic susceptibility pattern will help the clinicians in choosing appropriate antimicrobials for treatment which will reduce the major burden of septicaemia in critically ill patients and will also minimize the further emergence of resistance. Therefore, there should be an intensive surveillance, antibiotic policy formulations and preventive efforts for the effective management and prevention of drug resistance.