The Impact of Annona Muricata on Semen Quality and Antioxidants Levels in Alcohol Induced Testicular Dysfunction in Male Wistar Rats

The study was aimed to determine the impact of Annona muricata on biochemical and histological profile on alcohol induced testicular dysfunction in male Wistar Rat. Twenty five male Wistar rats with a weight of 130 160 grams were employed. Group A received feed and distilled water only, group B received 40 ml of Alcohol, and group C received 200mg kg of ethanolic leaf extract of Annona muricata ELAM . Groups D and E received 40 ml of Alcohol and were treated with 200mg kg and 400mg kg of ELAM for three weeks. The administration was done through oral gavage daily for three weeks. Data obtained for testosterone, Malondialdehyde MDA, Catalase CAT, gonado somatic index, and semen quality total sperm count, motility, and morphology were analyzed using ANOVA followed by post hoc LSD, and values were considered significant at p 0.05. The results showed a significant p 0.05 decrease in testosterone level, total sperm count, activity motility, and normal sperm cells in group B compared to A. However, in treatment groups, Annona muricata attenuated the effect on the parameters above, with a significant p 0.05 increase compared to group B. Alcohol toxicity caused an increase in abnormal sperm cells and gonado somatic index of the testes significantly p 0.05, which was attenuated by the Annona muricata. Also, significant higher MDA activities were observed in group B with no significant change in groups, C to E. CAT activities showed a significant decrease in group B and significantly attenuated CAT activities in group E. Histoarchitectural results revealed spermatogenic arrest and necropsied spermatids in group B, while moderate healing and increased spermatogenic process by the extracts in the treated groups. The study revealed testicular dysfunction resulting in infertility by alcohol toxicity however, Annona muricata showed improved fertility after reduced testicular function and improved CAT activities based on dose dependent with no effect on MDA level. Eyeghre, O. A. | Ezeokafor, E. N. | Dike, C. C. | Oparaji, K. C. | Amadi, C. S. | Chukwuma, C. C. | Ogbodo, O. N. | Obianyo, C. M. | Illah, C. | Nwoko, S. O. | Umeasiegbu, A. C. | Muorah, C. O. "The Impact of Annona Muricata on Semen Quality and Antioxidants Levels in Alcohol-Induced Testicular Dysfunction in Male Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-4, August 2023, URL: https://www.ijtsrd.com/papers/ijtsrd59798.pdf Paper Url:https://www.ijtsrd.com/medicine/physiology/59798/the-impact-of-annona-muricata-on-semen-quality-and-antioxidants-levels-in-alcoholinduced-testicular-dysfunction-in-male-wistar-rats/eyeghre-o-a