Quality Standardisation and Comparative Study of Leaf and Root of Kalanchoe pinnata in the Treatment of Wound Healing on Albino Rats

A wound is a disruption of the cellular and anatomic continuity of a tissue, with or without microbial infection. The perennial plant Kalanchoe pinnata (Bryophyllum) is a member of the Crassulaceae family. The present study was based on the quality standardisation and comparative study of leaf and root of Kalanchoe pinnata in the treatment of wound healing on albino rats. The plant was gathered in Uttarakhand, Dehradun, India. The plant leaves were used after being thoroughly rinsed in water. Additionally, the root was scrubbed and allowed to dry at room temperature in the shade and/or away from the sun. The plant was identified and authenticated by Dr Sunita Garg (Reference No. NIScPR/RHMD/Consult/2023/4486-87). The leaves and roots of Kalanchoe pinnata were extracted using methanol solvent. Physicochemical and phytochemical screening was done for both the extracts. The Methanolic extract of Kalanchoe pinnata root and leaf (5% Ointment Topical Application for 15 days) was assessed and compared for its wound healing activity on excisional wound healing animal using Albino rats. GC-MS analysis of the MEKP spectral spectrum showed, the presence of eight essential ingredients was shown as flavone with a retention time of 16.08 and a peak area of 11.9%, palmitic acid with a retention time of 16.73 and a peak area of 8.4%, and phytol with a retention time of 17.82 and a peak area of 39.8%. etc. It was found that MEKP Leaf (5% Ointment) showed superior contraction of wound and faster epithelialization compared to MEKP Root (5% Ointment). Our findings support the possibility of using this plant therapeutically in routine medical care. By following these recommendations, we can drive the development of safe, effective, and accessible phytoconstituent-based wound healing agents.