Study the Role of Guizotia Abyssinica Secondary Metabolites [GASM] on Gram Negative Bacteria and Human Citrated Plasma

The present study deals with the extraction, depiction and the role of Guizotia Abyssinica Secondary Metabolites [GASM] on gram negative bacteria and human citrated plasma. Guizotia Abyssinica seeds with holds numerous macro and micro nutrients which include vitamins, proteins, carbohydrates, minerals and mainly oil content. Guizotia Abyssinica oil mainly contains poly unsaturated and saturated fatty acids. Guizotia Abyssinica seeds were subjected to Sox-let-extraction with methanol and final crude was termed as GASM [Guizotia Abyssinica Secondary Metabolites]. GASM was initially screened was its biochemical content and also it was subjected to instrumentation method such as RP-HPLC and GC-MS. Interestingly both GC-MS and RP-HPLC peaks of GASM demonstrate that it should be presence of maximum amount of polyaromatic rings compounds like lipids, alkaloids, flavonoids, steroids and other secondary metabolites. In addition, plasma recalcification time was performed using Platelet Rich Plasma [PRP] and Platelet Poor Plasma [PPP] for GASM; unfortunately it doesn't show any activity like initiating the clotting process or delaying clotting process in both PRP and PPP. Apart from all this above mentioned assays, fortunately we found that non-toxic property of GASM when we perform non-toxic assay using packed RBC. Surprisingly, GASM exhibit anti-bacterial property against only gram negative bacteria