Eroepidemiology of Leptospirosis Among Healthy People in Zanjan, Northwest of Iran

Objective: A tropical disease of zoonotic origin, Leptospirosis is a public health concern, particularly in developing countries. This present investigation sought to determine the seroprevalence of Leptospira in healthy people referred to Shahid Motahari Laboratory, Zanjan, Iran. Methods: 181 healthy people referred to Shahid Motahari laboratory in Zanjan, Iran, were included in the present study. Serum samples were collected from each participant, whose demographic information was gathered using a questionnaire. After being stored -20 °C, the samples were analyzed for Anti-Leptospira IgG antibodies using NovaLisa Leptospira IgG ELISA" kit, the results of which were reported as positive, negative, or equivocal. Results: Out of 181 serum samples, 43 (23.7%) samples were positive, 36 (19.9%) were equivocal, and 102 (56.4%) were negative for anti-Leptospira IgG. The average age of the participants was 40.6 years old. No significant difference was shown between age and gender with anti-Leptospira IgG. IgG levels were not found to be correlated significantly with the rural residence of the participants, as well as the other demographic characteristics of participants. Conclusion: The results of this investigation contribute to the understanding of the epidemiological aspects of Leptospirosis in the region and underscore the importance of continued surveillance and awareness efforts.