Tumor Necrosis Factor Affects Steroidogenic Factor-1 Expression in Ovarian Granular Cells Via Inducing Gene Methylation |Biomedgrid

Objective: The expression of SF-1 in ovarian granulosa cells is critical for follicular growth and development, and the decreased expression of SF-1 in ovarian granulosa cells under endometriosis (EMs) pathology is not clear. This study aims to investigate the mechanism of tumor necrosis factor (TNF), a factor promoting endometriosis, in regulating the promoter methylation and expression of steroidogenic factor-1 (SF-1) in ovarian granulosa cells. Methods: Human ovarian granulosa cell line KGN was used. Methylation inhibition was mediated by cells treated with 5-Aza-2 ‘deoxycytidine (5-Aza). The inhibition of the p65 expression gene in KGN cells was mediated by a lentiviral CRISPR/Cas9 vector (p65- /-KGN cells). The methylation level in the promoter region of SF-1 was detected by BSP assay and SF-1 expression was determined using quantitative real-time PCR and western blot.