The Effects of Cannabinolic Acid Amides on TNF-Α and IL-6 Expression in Lipopolysaccharide Activated Human Peripheral Blood Mononuclear Cells |Biomedgrid

Introduction: The effects of tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA) amide derivatives 1-9 on TNF-???? and IL-6 expression and cell proliferation were investigated on lipopolysaccharide (LPS) activated human peripheral blood mononuclear cells (PBMCs). Materials and Methods: Compounds 1-9 were tested on LPS activated human PBMCs and several inflammatory endpoints monitored. Negative controls included PBMC only, LPS and dexamethasone with LPS. After a 72-hour incubation, PBMC supernatants were collected to measure IL-6 and TNF-α levels by ELISA. Cell pellets were harvested, and cell proliferation analyzed with the CTG assay. Results: Compounds 1 and 2 increased TNF-α expression and inhibited cell proliferation, while unsubstituted amides 5, 6 decreased the production of TNF-α compared to LPS-treated cells. None of the compounds tested had a noticeable effect on LPS induced IL-6 expression.