Potential Diagnostic Biomarkers for Human Uterine Mesenchymal Tumours Especially LMP2 1i and Cyclin E1 Differential Expressions

Aims Although the majority of smooth muscle neoplasms found in the uterus are benign, uterine leiomyosarcoma is extremely malignant, with high rates of recurrence and metastasis. The development of gynecologic tumors is often correlated with secretion of female hormone however, the development of human uterine leiomyosarcoma is not substantially correlated with hormonal conditions, and the risk factors are unclearly understood. Importantly, a diagnostic biomarker, which distinguishes malignant human uterine leiomyosarcoma from benign tumor leiomyoma is yet to be established. It is necessary to analyze risk factors associated with human uterine leiomyosarcoma, in order to establish a diagnostic biomarker and a clinical treatment method. Methodology Histology and Immunofluorescence Staining tissue sections 5 µm were prepared and stained with HandE for routine histological examination or were processed further for immunofluorescence staining with appropriate antibodies. Furthermore, a total of 57 patients between 32 and 83 years of age and diagnosed as having smooth muscle tumors of the uterus were selected from pathological files. Immunohistochemistry staining for LMP2 1i and cyclin E1 was performed on serial human uterine leiomyosarcoma, leiomyoma and myometrium sections. Results Homozygous deficient mice for a proteasome subunit LMP2 1i spontaneously develop uterine leiomyosarcoma, with a disease prevalence of ~40 by 14 months of age. Defective LMP2 1i and cyclin E1 positive expressions in human uterine leiomyosarcoma were demonstrated, but the reverse result was obtained in human leiomyoma and myometrium. Conclusions LMP2 1i and cyclin E1 differential expressions may be one of the risk factors for human uterine leiomyosarcoma. LMP2 1i and cyclin E1 may be potential diagnostic biomarker and targeted molecule for a new therapeutic approach. Takuma Hayashi | Hiroyuki Aburatani | Ikuo Konishi "Potential Diagnostic Biomarkers for Human Uterine Mesenchymal Tumours: Especially LMP2/1i and Cyclin E1-Differential Expressions" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd62380.pdf Paper Url: https://www.ijtsrd.com/medicine/other/62380/potential-diagnostic-biomarkers-for-human-uterine-mesenchymal-tumours-especially-lmp21i-and-cyclin-e1differential-expressions/takuma-hayashi