Environmentally friendly energy, extremophilic microorganisms, enzymatic activity, microbial fuel cell, hard-to-decompose substrates
Journal: Техника и технология пищевых производств (Food Processing: Techniques and Technology) (Vol.54, No. 1)Publication Date: 2024-03-29
Authors : Anastasia I. Dmitrieva; Elizaveta R. Faskhutdinova; Timothy A. Larichev; Natalia S. Velichkovich; Veronika Yu. Boger; Larisa M. Aksenova;
Page : 27-36
Keywords : Environmentally friendly energy; extremophilic microorganisms; enzymatic activity; microbial fuel cell; hard-to-decompose substrates;
Abstract
Traditional energy sources pollute the environment. Microbial fuel cells are an alternative energy source that can reduce the environmental burden. Microbial fuel cells also remove recalcitrant wastes from wastewater. This research featured the enzymatic potential of microbial isolates obtained from the Abakan Arzhan thermal spring. The study involved isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas. The keratinolytic analysis was in line with State Standard R 55987-2014. The chitinolytic activity was determined by injecting a bacterial suspension on Petri dishes with a chitin-containing medium. The lipolytic analysis involved cultivating the isolates in Stern’s glycerol fuchsin broth. The xylan hydrolysis depended on the reducing sugars. The cellulase activity was measured according to the standard method recom mended by the International Union of Pure and Applied Chemistry (IUPAC). The catalase potential was evaluated by the gasometric method on 1% gasoline media. The optimal parameters of consortium cultivation were determined by the voltage generated. The Geobacter isolate had the maximal keratinolytic activity while the Thermomonas isolate demonstrated the maximal protein hydrolysis (80.1 ± 1.5%). Both Geobacter and Rhodopseudomonas showed good lytic activity against chitin with the lysis zone of ≥ 3 mm. The Geobacter isolate demonstrated as many as 350 units of xylanase activity and 365 units of cellulase activity; Ther momonas had 350 units of xylanase activity and 360 units of cellulase activity; Rhodopseudomonas showed 310 units of xylanase activity and 304 units of cellulase activity. The maximal catalase properties belonged to Geobacter (1.40 units) and Thermomonas (1.38 units). The maximal energy generation by bacterial consortia occurred at pH 8 and 45°C after 48 h of cultivation. In this research, isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas from the Abakan Arzhan thermal spring were able to remove recalcitrant components, thus demonstrating good prospects for biological treatment of industrial wastewater.
Other Latest Articles
- Effect of Wine and Grape Spirits from Table Grape Varieties on Sensory Profile of Grape Beverages
- Quality Assessment of Young Sparkling Wines of Crimean Indigenous Grape Varieties
- Examining The Factors That Influence Indonesia’s Halal Tourism Destination Brand Equity
- Analisis Bibliometrik Penerbitan Prestasi Institusi Kewangan Islam dalam Pangkalan Data Scopus
- A Preliminary Investigation: Examining The Utilisation of Islamic Fintech Credit by Asnaf Microentrepreneurs, Their Access to Microfinance Through Zakat Institutions, and its Impact on Income and Subjective Well-Being
Last modified: 2024-04-05 13:02:48