Antioxidant and Anticancer Effects of Thymoquinone on Colorectal Cancer Cells |Biomedgrid

Colorectal Cancer (CRC) is one of the most leading causes of death in the world and one of the major public health problems. Despite the great advances in cancer therapy, the incidence and mortality rates of CRC remain high. Therefore, there's a need for more efficient and less toxic cancer treatment strategies. Herbs and spices could provide potential anticancer candidates. Herein we investigate antioxidant and anticancer activities of thymoquinone (TQ). Anticancer activity against HCT116 and HT29 was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays while antioxidant activity was assessed by DPPH and ABTS tests. For apoptosis study, Hoechst test were used. TQ induce HCT116 and HT29 cell cytotoxicity and apoptosis without affecting HCEC, non-tumoral cells. This study is the main step to develop a highly active and multicomponent anticancer drug from natural sources.