A New RP-UPLC Method for Simultaneous Quantification of Ivabradine and Metoprolol in Combined Dosage Forms

A simple, sensitive, precise, accurate and reportable method was developed for Ivabradine and Metoprolol succinate using a simple phase UPLC system. The chromatographic system consisted of a Shimadzu Nexera XR UPLC chromatograph equipped with a Hibar C18 (100 X 2.1 mm, 2 μ) column, FCV-32AH pumps and an SPD-20A photo diode array (PDA) detector. The mobile phase used was Water and Methanol in the ratio of 70:30. The flow rate was found to be 0.5mL min -1 and the detection wavelength was 260nm. The retention times of Ivabradine and Metoprolol were found to be 1.197 and 1.628min, respectively. The detection limits for Ivabradine and Metoprolol were found to be 0.06 and 0.28 μg mL-1, respectively. The limit of quantification (LOQ) for Ivabradine and Metoprolol was found to be 0.19 and 0.85 μg mL-1, respectively. This method has been validated according to ICH guidelines and can be used to evaluate drug purity in bulk and dosage forms.