ANALYSIS OF ASSOCIATION BETWEEN TLR-4 ASP299GLY AND THR399ILE GENE POLYMORPHYSIMS AND CHRONIC PERIODONTIS IN BABYLON POPULATION, IRAQ

To analyze the association between toll like receptors-4 asparginine299glycine and therionine399ilucine gene polymorphisms and chronic periodontitis.Genomic DNA was obtained from peripheral blood of 40 patients with chronic periodontitis and 30 periodontally healthy controls. TLR-4 Asp299Gly and Thr399Ile gene polymorphisms were genotyped by a polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed by calculation of Odds ratio (OR) with 95% confidence interval (95%CI) using logistic regression analysis. Statistical significance was set at a p value? 0.05.Theallele's frequency of Thr399Ile gene was higher among CP group (82.5%) than control (63.3%) with significant difference. The prevalence of a Thr399Ile heterozygote was found to be 10% in the chronic periodontitis group and zero in the periodontally healthy group.There was significant association between receptor- 4 therionine399ilucine and chronic periodontitisand no significant difference in the toll like receptor-4 asparginine299glycinegene polymorphism between the chronic periodontitis and controls.