NOVEL METHOD FOR SYNTHESIS OF MELANIN BASED SILVER NANOPARTICLES AND ITS APPLICATION AGAINST STAPHYLOCOCCUS AUREUS

A group of biopolymers is known as melanin, melanin is getting more attention due to its applications in diverse field such as cosmetics, bioremediation, and pharmaceutical industries. Melanin can be biosynthesized via bacteria and fungi. In present study melanin producing fungal strain was procured from repository and Production of melanin was carried out by submerged fermentation using tyrosine casein medium. Extraction and Characterization of melanin was carried out using various test like bleach test, solubility test, reaction with ferric chloride, reaction with hydrogen peroxide, thin layer chromatography and FTIR spectroscopy. Extracted melanin was used for synthesis of silver nanoparticles. Colour change after incubation of melanin with silver nitrate indicated formation of silver nanoparticles, further synthesized nanoparticles was characterized by UV-Visible spectroscopy, Dynamic light scattering and zeta potential analysis. Antibacterial activity of melanin based synthesized nanoparticles was found against Staphylococcus aureus. Melanin based synthesized nanoparticles can be proved as stable and eco friendly nature and having wide range of applications in fields like agriculture, textile, bioremediation, food coating, bio imaging, nanomedicine, and various pharmaceutical drugs.