Multidrug Resistance Proteus spp. in Meat and their Evaluation against Nano-Emulsion

In the present evaluation, Proteus spp. isolated from the meat samples collected from the slaughterhouses were screened for their antibiotic susceptibility against Amikacin, Ampicillin, Ciprofloxacin, Colistin, and Gentamicin. Among the isolates, strain PBM32, showing a higher resistance toward most of the antibiotics was identified as Proteus mirabilis (NCBI Accession Number OR398497). As an alternative to the antibiotics, nanoemulsion was evaluated and it has significantly reduced (46% reduction) the motility of P. mirabilis PBM32 with respect to the control, and reduced the biofilm formation by 71.4%, 50%, and 55.5%, with respect to control, preservative and antibiotic treatments, respectively. Nanoemulsion treatment recorded a significant reduction of P. mirabilis PBM32 population in the pork samples and a reduction in mutton and beef samples when compared to other antimicrobials.