Antihyperglycaemic Constituents from Cleistopholis Patens and Sansevieria Liberica as Justification of their Antidiabetic Ethnomedicinal Claims |Biomedgrid

The antihyperglycaemic activity of the methanol leaf and rhizome extracts of Cleistopholis patens and Sanseviera liberica, respectively has been reported and their dichloromethane and n-hexane partitioned fractions, respectively were found to be active. This study therefore evaluated the antihyperglycaemic activity of the chromatographic column fractions of the dichloromethane and n- hexane partitioned fractions of Cleistopholis patens and Sanseviera liberica, respectively. The glucose lowering constituents of the active subfractions were also isolated thereby further justifying their antidiabetic ethnomedicinal usage in Nigeria. The active dichloromethane and n-hexane fractions of methanol leaf and rhizome extracts of Cleistopholis patens and Sanseviera liberica, respectively were subjected to column chromatography. The subfractions obtained were assayed for antihyperglycaemic effect using glucose-induced hyperglycaemic model with glibenclamide (5 mg/kg) and 1 % Tween 80 in normal saline as positive and negative controls, respectively. The most active column fractions of the two plants were further purified by various chromatographic separations which led to the isolation of their antihyperglycaemic constituents. The results of the study showed that subfractions C2, C4, C5, and C8 of C. patens at 400 mg/kg gave comparable antihyperglyceamic activity to glibenclamide (5 mg/kg). Also, subfractions C2, C4, C8 and C9 of the n-hexane fraction of S. liberica were comparable in activity and gave comparable and similar profile of activity to glibenclamide (5 mg/kg). The structural elucidation of the isolated β-sitosterol from C. patens and β-stigmasterol from S. liberica that were carried out using 1D and 2D NMR spectra and their NMR data compared well with literature. The study concluded that the β- sitosterol from Cleistopholis patens and β-stigmasterol from Sanseviera liberica were some of the antihyperglycaemic constituents of the plants and thus justified their folkloric antidiabetic usage in Nigeria.