EVALUATION OF TOTAL PHENOLIC, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITIES OF CROCUSCHRYSANTHUS(HERB.) HERB. WITH CONVENTIONAL SOXHLET EXTRACTION
Journal: International Journal of Advanced Research (Vol.12, No. 08)Publication Date: 2024-08-14
Authors : Cihan Dusgun; Ali Keskin;
Page : 641-647
Keywords : C.chrysanthus Antioxidant DPPH CUPRAC FRAP;
Abstract
Species belonging to the genus Crocus L. have some traditional uses against some cardiovascular diseases, diabetes, Parkinsons disease, depression, apoptosis, atherosclerosis. In this study, total phenolic, total flavonoid and antioxidant potentials of the plant extract obtained using Crocus chrysanthus(Herb.) Herb.were investigated. C. chrysanthus was extracted with 70% ethanol using a soxhlet apparatus. While total phenolic content was found to be 127.48 mg gallic acid equivalent (GAE/g), total flavonoid content was found to be 41.67 mg quercetin equivalent (QE/g). Radical scavenging (DPPH) and metal chelation (CUPRAC and FRAP) methods were used to determine the free radical scavenging properties. While DPPH radical scavenging was found to be 85.27%, CUPRAC was found to be 56.83 mg trolox equivalent (TE/g). In the FRAP method, the reducing power was found to be 57.68trolox equivalent (TE/g). According to the results obtained, it was determined that the ethanol extract of C. chrysanthus exhibited antioxidant properties. In addition, phenolic and flavonoid content was determined. As a result, C. chrysanthus extract can be recommended as a source of bioactive components with potential use against chronic diseases caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of compounds isolated from C. chrysanthus to determine the main compounds modulating the observed activities.
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