PHYSIOLOGICAL AND MOLECULAR ASPECTS OF HEART OF RAT FED WITH CHOLESTEROL REACH DIET - THE IMPACT OF PROCAINE TREATMENT

The aim of this study was to investigate the effect of ischemia reperfusion(I/R) upon cardiac physiological parameters(CF) coronary flow,(HR) heart rat as well as LVPD(Left ventricle pressure developed) as well as on apoptosis in adult Wistar rats feed on Cholesterol diet and treated with Procaine. Material and methods:18 male Wistar rats aged 12 months have been used in our experiment divided into 3 groups of 6 rats each: group A Controls, Group B Cholesterol feed rats, group C Cholesterol feed rats treated with Procaine (20mg/kg body weight for 8 weeks). High cholesterol diet (lard mixed with chew) has been used to feed rats for 8 weeks. At the end of treatment the hearts have been excised and mounted in Langendorff reperfusion system. A 45 minutes ischemia has been followed by 120 minutes reperfusion on isolated rat heart in order to measure heart rate, coronary flow and left ventricle developed pressure as well as to assay left ventricle for apoptosis .Our data have pointed out modifications in physiological parameters and the presence of apoptosis in cholesterol treated rats while the Procaine seems to have a benefic effect on these parameters and on DNA integrity.