Short-Term Use Of Cell Phones Has No Significant Effect On The Salivary Oxidant/Antioxidant Profile

To investigate whether or not low intensity radio frequency electromagnetic field exposure (RF-EME) associated with cell phone use can affect human cells, the present study was carried out. Saliva samples collected before using a cell phone as well as at the end of 15 and 30 min calls were tested for two commonly used oxidative stress biomarkers. The 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxo-dG) levels were determined by enzyme linked immunosorbent (ELISA) competitive assay. The malondialdehyde (MDA) levels were measured using the OxiSelect MDA Adduct ELISA Kit. The antioxidant capacity of the saliva was evaluated using the oxygen radical absorption capacity (ORAC) and the hydroxyl radical averting capacity (HORAC) assays. The mean 8-oxodG and the Bradford protein concentrations (ng/ml and mg/ml, respectively) peaked at 15 min. The levels of HORAC, ORAC and MDA progressively increased with time and reached maximum at 30 min. All measured parameters were not significant when values before and after talking are compared indicating that serious changes in the salivary oxidant/antioxidant profile may not be strongly correlated with exposure to RF-EME.