Collagen matrix with increased hemostatic properties: comparative analysis of hemostatic efficacy

Aim. To develop collagen matrices with increased hemostatic properties and to evaluate their effectiveness in comparison with commercial products. Methods. Collagen was obtained from calfskin by acid hydrolysis, identified by SDS-PAGE, and sterilized in an autoclave. The enzymatic clotting activator was purified from Echis multisquamatis venom by ion exchange chromatography. The activity of the activator was evaluated with the chromogenic substrate S2302. Ready-made modified matrices were obtained by lyophilization. Hemostatic efficacy was tested in the Wistar Han rat hepatic hemorrhage model, compared with commercial materials. Results. Optimal concentrations of collagen (300 μg/cm2) and enzyme activator (10 μg/cm2) were selected for the manufacture of collagen matrices with increased hemostatic properties. Studies on the rat parenchymal bleeding model showed that these matrices provide faster bleeding control compared to commercial hemostatic materials. After the removal of collagen matrices, bleeding did not resume. Conclusions. The developed collagen matrices with increased hemostatic properties demonstrate high efficiency, stable clot formation, and minimal risk of rebleeding, which confirms their prospects in surgery.