Effect of resveratrol on regulated cell death of enterocytes under conditions of oxidative stress in vitro

Aim. The study purposed to evaluate a concentration-dependent effect of Sirtuin 1 (SIRT1) modulators on cell death of enterocytes in the ileum of the small intestine under conditions of oxidative stress. Materials and Methods. The experiments were conducted using female Alba mice. Isolation of enterocytes was carried out according to Kimura et al. 2015. Modeling of oxidative stress (OS) by adding hydrogen peroxide to phosphate-buffered saline: “weak” (50 μM); “medium” (250 μM); “strong” (500 μM). Apoptotic and necrotic death of enterocytes was assessed by morphological characteristics using the in vivo method of the two-color fluorescent nucleic acid dye Hoechst 33342 and propidium iodide. Substances used: Resveratrol — SIRT1 activator and Ex527 — specific SIRT1 inhibitor (2 μM, 20 μM, 100 μM). Results. An increase in the number of live cells (P < 0.01) and a decrease in apoptotic (P < 0.01) and necrotic (P < 0.01) cells was observed with increasing Resveratrol concentration (from 2 to 200 μM) under OS conditions. Conclusion. The obtained results suggest that Resveratrol reduces cell death of enterocytes, involving activation of SIRT1 under oxidative stress conditions in vitro. Further research is needed to confirm the effectiveness of this strategy and to establish protocols for the inclusion of antioxidants in treatment regimens for inflammatory bowel disease.