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IDENTIFICATION OF GRAM?NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

Journal: Fisheries Science of Ukraine (Vol.23, No. 1)

Publication Date:

Authors : ;

Page : 80-86

Keywords : PCR-RFLP; 16s rRNA; gram?negative (G-) bacteria;

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Abstract

The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S rDNA gene of gram-negative bacteria and also possibility of differentiation of these bacteria by use of the restriction endonuclease sets were shown.

Last modified: 2015-06-15 22:02:48