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USE OF MULTIPLEX POLYMERASE CHAIN REACTION IN RAPID DIAGNOSTICS OF INFECTIOUS DISEASES OF FISH
Journal: Fisheries Science of Ukraine (Vol.21, No. 3)Publication Date: 2012-09-25
Authors : Rud Yu.; Zhuk L.; Vladymyrsky I.;
Page : 59-62
Keywords : multiplex polymerase chain reaction; Renibacterium salmoninarum; Yersinia ruckeri; Flavobacterium;
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- USE OF MULTIPLEX POLYMERASE CHAIN REACTION IN RAPID DIAGNOSTICS OF INFECTIOUS DISEASES OF FISH
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Abstract
The method of multiplex polymerase chain reaction for simultaneous detection of major bacte- rial fish pathogens Renibacterium salmoninarum, Yersinia ruckeri, Flavobacterium columnarae was developed. The specificity and effectiveness of oligonucleotide primers and also the advantages of developed method in comparison with bacterial examination were shown.
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