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Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Journal: Baghdad Science Journal (Vol.12, No. 2)

Publication Date:

Authors : ; ;

Page : 332-339

Keywords : Thymol; Flow-injection Spectrophotometric determination; 4-Aminoantipyrine; copper nitrate.;

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Abstract

Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations. The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm. Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 μg.ml-1 of thymol . The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 μg mL-1 and 3.60 μg mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations. The procedure is characterized by its simplicity, accuracy and precision.

Last modified: 2015-06-20 17:58:30