Production Of Pullulanase Using Novel Organic Substrates

Abstract Production of pullulanase was carried out from I. batatas P. vulgaris E. crassipes and P. stratiotes using A. pullulans. The fermentation reactions were carried out in minimal basal media at 28C. E. crassipes and P. stratiotes as substrates produced more pullulanase than I. batatas and P. vulgaris. The highest enzyme production from Pistia stratiotes was of 9.3 Uml after 72 hours of incubation at 28C and Eichhornia crassipes showed most enzyme production of 4.65 Uml after 48 hours of incubation at 28C. The two novel substrates E. crassipes and P. stratiotes could successfully be used for the production of pullulanase and are a promising resource for large-scale production of the same.