DEVELOPMENT AND VALIDATION OF NOVEL UVSPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF DONEPEZIL IN BULK AND PHARMACEUTICAL DOSAGE FORM

The simple, precise and cost effective spectrophotometric methods have been developed for the estimation of Donepezil in bulk and its pharmaceutical formulations. The absorption maximum was found to be at 314nm in Zero order derivative spectrum (Method A) with the correlation coefficient 0.9988 and for the calculation of Area Under Curve (AUC)(Method B) wavelength range was found to be 304-324nm, with the correlation coefficient 0.9985. The drug follows the Beer-Lambert’s law in concentration range of 4-20?g/ml for both the methods. The solvent used is methanol: water in the ratio 30:70 for the preparation of stock solution. The methods validated by analytical parameters and all the validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Donepezil in bulk and pharmaceutical dosage forms. Key Words: Donepezil, Area under Curve, Zero order derivative spectroscopy, Validation, Correlation Coefficient.