Preparation and Characteristic of Dextran-BSA Antibody and Establishment of it’s Elisa Immunoassay

The enzyme linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextran?BSA antigens were prepared by Reductive amination method, and were confirmed by SDS-PAGE and free amino detection. The effects of coupled reaction conditions such as different oxidation degree of dextran, the reaction time were investigated and the immunity of the resulting dextran- BSA neoglycoprotein antigens were evaluated through the interaction with standard dextran antibody. The immunogen was immunized with white rabbits to obtained polyclonal antibody respectively. A general and broad class-specific Elisa detection method was developed according to Elisa theory. The method was put to use for quantitative analysis of dextran in practical saccharose samples.