PHENOTYPIC AND GENOTYPIC INVESTIGATION ON SILVER NANOPARTICLES OF MORGANELLA MORGANII RECOVERED FROM (CAUTI), IRAQ

One hundred eighty eight mid-stream urine samples were collected from patients suffering from Catheter Associated Urinary Tract Infections, (CAUTI), who visit urological consultant clinic of Hillah Teaching Hospital in Hillah, Babylon province- Iraq during a period from November 2012 to January 2013. All urine samples were subjected for standard bacteriological procedures to check bacteriuria. The results show that 9/159 (5.66%) give positive culture for Morganella morganii. For detection the ability of this bacteria to produce silver nanoparticles we are used the XRD the result of XRD for the control shows amorphous phase but after loading the silver nitrate (AgNO3) on the surface of the isolates we get new peacks (111,200,220,311) for isolates number 2, 3, 4,5,6,7 and the isolates and the isolate number 1,8 and 9 show negative result (the amorphous phase). These peaks approved that silver ions doping on the surface and this confirmed by using TEM. The UV-VIS used to study the behavior of silver nanoparticles this shows it is clear at zero hour there is no reaction of her and at 24 ,72 and 120 the increased in intensity will be observed in the range of (400 & 500)nm.