BT GENE AND BT PROTEIN STUDIES IN TULASI COTTON HYBRIDES BY PCR AMPLIFICATION.

Cotton, the most prevalent fiber used in the clothing today, often referred as the White Gold. It contributing up to 75% of total raw material needs of textile industry. But, cotton crop is highly susceptible to insects; especially to the larvae of lepidopteron pests, which is impacting cotton production. Of these pests, the American boll worms alone cause yield reduction up to 40 ? 70 % under severe incidence. it has been argued that adoption of Bt cotton could help in protecting the crop against potentially the most damaging bollworms and thus reduce the risk of crop failures and the use of insecticides leading to create eco-friendly environment without compromising on profitable yield. a transgenic plant, produces an insect controlling protein Cry1A(c), the gene for which has been derived from the naturally occurring bacterium, Bacillus thuringiensis subsp. Kurstaki. The Present studies aims at identifying the Bt gene and Quantifying the Bt protein in three different Tulasi Bt cotton hybrids TCHH-4, TCHH-9, TCHH-17.This involve isolating the DNA from the Hybrids and amplifying the Bt gene by PCR amplification and Testing the Zygosity of the Bt gene in the plant hybrid using Gel electrophoresis