TARGETING EFFICIENCY EVALUATION OF FLUOROURACIL IN PLGA MICROSPHERES CONJUGATED WITH HUMAN MONOCLONAL ANTIBODIES

In the advent of producing an effective tumour targeting drug system, Poly lactic acid-co-glycolic acid microspheres were formulated using Double (multiple) emulsion technique with and without human Ig G. The ideal ratio of drug to albumin was attained by varying the concentration of drug and this ideal batch of drug loaded microspheres was selected and subjected to physical characteristics such as size, shape and uniformity by size distribution analysis. All the test batches of microspheres were subjected to in-vitro evaluation and ideal batch of 5-Fluorouracil microspheres with and without monoclonal antibodies were subjected in-vivo evaluation for drug distribution and targeting efficiency by comparing with free 5-Fluorouracil. The results helped to arrive for a conclusion that the Fluorouracil immune-microspheres group was more effective than Fluorouracil microspheres and Fluorouracil groups but it will be worthwhile to investigate the anticancer activity of active targeting system by conjugating specific antibodies against to specific tumour antigen.