A NOVEL RP-HPLC METHOD FOR THE QUANTIFICATION OF RUXOLITINIB IN FORMULATIONS

A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Ruxolitinib in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted of Acetonitrile: Water: Tetra Hydro Furan (THF) 60:30:10% (V/V/V). The UV detection wavelength was 227nm and 20μl sample was injected. The retention time for Ruxolitinib was 4.28min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Ruxolitinib in tablet dosage form and bulk drug.