Langerhans Cell - The Destination of Migration

Langerhans cells (LCs) capture irritant substances in the skin, and then LCs migrate to the lymph nodes for presenting antigens. For investigating this, we used mites antigen labeled with PKH26 dye in atopic model NC mice for patch testing. The readings of skin reactions were done at 0.5, 1, 3, 6, 9, 24, 48, 72 and 168 hours after the patch testing. At 24-, 48-, 72- and 168-hour, some LCs expressed PKH26 dye were observed in the sinus, T-cell area and marginal area of the regional lymph nodes. The B-cell areas of these lymph nodes were shown as dark and round-shaped at the same time points. By electron microscopy, we found rod-shaped BGs (atypical granules) in the Golgi area of these lymph nodes. We recognized again and showed that LCs with antigen migrate to the lymph nodes.