AN INVESTIGATION OF ANTIOXIDANT AND CYTOTOXIC PROPERTIES OF GREEN SYNTHESIZED SILVER NANOPARTICLES

Silver nanoparticles are being used in numerous fields for their optical, conductive, antioxidant anticancer and antibacterial properties. Green synthesis of silver nanoparticles is a simple and reliable method over chemical, physical and mechanical methods. The leaf extract of Aegle marmelos was successfully used for the synthesis of silver nanoparticles. Use of the silver nanoparticles in the field of biomedicine is rapidly growing because of their high efficacy and low toxicity. With respect to biological and clinical applications, silver nanoparticles has ability to control and manipulate the accumulation of nanoparticles inside a cell can provide sensitivity towards diagnosis and therapeutic efficacy. Here the antioxidant property of silver nanoparticles studied by performing 1,1-diphenyl- 2-picryl hydrazyl radical, nitric oxide and hydrogen peroxide scavenging assay. While cytotoxicity of the biogenic silver nanoparticles studied by performing the MTT assay. The result obtained for biogenic silver nanoparticles shows the dose dependent increase in antioxidant activity and it is comparable with standard ascorbic acid. Silver nanoparticles exhibits cytotoxic activity against A-431 squamous cell carcinoma cell lines. This potential to silver nanoparticles may impart form capping agent from leaf extract. Study of the anticancer mechanism of biogenic silver nanoparticles is the further way of research in coming future. Keywords: Biogenic silver nanoparticles, antioxidant, cytotoxicity, squamous cell carcinoma, A431 cell lines.