Development and Validation for the Simultaneous Estimation of Moxifloxacin Hydrochloride and Bromfenac Sodium by RP- HPLC

In the present study an HPLC method was developed and validated for the simultaneous determination of Moxifloxacin Hydrchloride and Bromfenac Sodium. The chromatographic system was equipped with SULPELCO 516 C-18 column and UV detector set at 275 nm, in conjunction with a mobile phase of 0.05 M Potassium Dihyrdrogen Phosphate (pH 4.5) and Acetonitrile (60:40%V/V) at a flow rate of 1.0 ml/min and the injection volume set at 20 ìl with 10 minutes of runtime. The described method was linear over a concentration range of 5.5-27.5 ìg/mL for MOX and 1-5 ìg/mL for BROM with a with good linearity response of 0.9996 and 0.9994 respectively. The retention time of Moxifloxacin Hydrchloride was 3.970 min ± 0.29and of Bromfenac Sodium was 5.000 min ± 0.15. The results of analysis were validated. The results of the study showed that the proposed RP-HPLC method was simple, rapid, precise and accurate, which is useful for the routine determination of Moxifloxacin Hydrochloride and Bromfenac sodium in bulk drug and in pharmaceutical dosage form.